1. The liberated choline is taken up again by the pre-synaptic neuron and ACh is synthesized by combining with acetyl-CoA through the action of choline acetyltransferase.

결합으로부터 해방된 choline은 pre-synaptic neuron에 의해 재흡수되고, choline acetyltransferase의 작용을 통해acetyl-CoA와 결합함으로써 ACh가 합성된다.