1. However, the restriction enzyme digestions identified two different genomic DNA modifications in Acetobacter.

2. Thermo Scientific SgsI (Asci) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer

3. Thermo Scientific Eco88I (Avai) restriction enzyme recognizes C^YCGRG sites and cuts best at 37°C in in Tango buffer

4. Thermo Scientific Boxi (PshAI) restriction enzyme recognizes GACNN^NNGTC sites and cuts best at 37°C in Tango buffer (isoschizomers: BstPAI, PshAI)

5. 26 Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.

6. In the third schematic, the probe and restriction enzyme are chosen to detect a region of the genome that includes a variable number tandem repeat segment (boxes in schematic diagram).

7. In an attempt to study Giardia at the DNA sequence level, the rRNA genes of three species, Giardia duodenalis, Giardia Ardeae and Giardia muris were cloned and restriction enzyme maps were constructed

8. The invention relates to a method called Restrictase Chain Reaction (RCR) for directly detecting target nucleotide or target amino acid sequences or target antigens by amplification of a nucleotide sequence using DNA polymerase, a restriction enzyme and a matrix DNA, on which an initial primer is bonded and amplified.