1. Wear ligating modules, headgear and other accessories as instructed by your orthodontist.

2. The present disclosure provides self-ligating orthodontic brackets with improved control over access to the archwire slot.

3. The invention provides novel reagents and methods for ligating an acyl donor compound with an acyl acceptor compound.

4. Cloning involves digesting the vector and DNA fragments, purifying them, ligating them with one another, and transforming the wild mixture that emerges within the bacteria.

5. Methods:The animal model of acute myocardial ischemia was reproduced in anesthetized dog by ligating the left coronary artery at the middle of anterior descending branch.

6. PCR Cloning offers some advantages over traditional Cloning which relies on digesting double-stranded DNA inserts with restriction enzymes to create compatible ends, purifying and isolating sufficient amounts, and ligating into a similarly treated vector of choice (see insert