1. The Antiserum is then tested by immunodiffusion or immunoelectrophoresis for its ability to form immunoprecipitate

2. 7 We will conduct double agar diffusion test,[www.Sentencedict.com] simple agar diffusion test and counter immunoelectrophoresis with aluminium potassium sulfate and sodium bicarbonate.

3. As examples, methods that may contribute to this include electrophoresis (SDS-PAGE, immunoelectrophoresis, Western blot, isoelectrofocusing), high-resolution chromatography (e.g., reversed-phase chromatography, gel filtration, ion exchange, affinity chromatography), and peptide mapping.

4. Other than these methods, the techniques to separate protein antigens, namely, polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional immunoelectrophoresis, western blotting, dot blotting, and immunobinding assay, have also been developed and attempted to diagnose Caprine agalactia [34, 35, 40, 99-103].