1. (a) Representative whole-mount alexa-fluor-488 phalloidin-stained preparations from basal turns of Cochleae

2. Example: Use secondary antibodies anti-rabbit Alexa Fluor® 488 pre-Adsorbed against mouse serum and an anti-mouse Alexa Fluor® 647 pre-Adsorbed against rabbit serum when performing a double staining experiment using primary antibodies raised in rabbit and mouse respectively.

3. The green-fluorescent Alexa Fluor® 488 Azide is reactive with terminal alkynes via a copper-catalyzed click reaction

4. The images show merge of annexin A2 and Alexa-Fluor-488 Actinfrom control C25 and dysferlinopathy DYSF3 myoblasts shown inFigure 5A

5. Alexa Fluor® 555 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety

6. Alexa Fluor 488 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety

7. The normalKirsanov reaction of fluor-substituted anilines yields fluorinated N-phenyl-trichlorophosphineimines, which on ammonolysis give the corresponding bis(phosphoranylidene) ammonium chlorides [RNH−P(NH2)2]2N+Cl−.

8. In preliminary experiments, mouse anti-calpastatin antibodies (Mab) were conjugated to Acceptor dyes (Alexa Fluor 594), while the second binding agent (calpain I) was conjugated to …

9. Atmin Antibody (E-12) is available as both the non-conjugated anti-Atmin antibody form, as well as multiple conjugated forms of anti-Atmin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor ® conjugates

10. WarmedPBS,permeabilized,andlabeledwith0.4 MActinfrom rabbit muscle conjugated to Alexa Fluor 568 in permeabiliza-tion buffer (20 mM HEPES, 138 mM KCl, 4 mM MgCl 2,3mM EGTA, 0.2 mg/ml saponin, 1% BSA) plus 1 mM ATP for 15 s at 37 °C