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2. Protoplast-derived cells developed calli on transfer to agar-solidified medium.

3. The origin of the midvein calli developed mainly from the vascular cylinder, the palisade tissues and the phloem parenchyma cells.

4. 15 When the obtained calli were further transferred onto MS medium supplemented with 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP),[www.Sentencedict.com] some of them regenerated plants.

5. The results showed that calli inducing adventitious roots and Apicals for 30 days obtained the highest yield of flavonoid, indicating effective production for flavonoids instead of wild culture

6. “In my research it was found that many times school Bullies continue the harassment online,” says Calli Tzani-Pepelasi, an investigative psychology lecturer at the University of Huddersfield.