1. Investigators hypothesized that if Anergy is a credible process to explain tolerance to self, anergic cells would persist but would fail to respond to antigenic stimulation.

2. Anergy skin testing and preventive therapy for HIV-infected persons: revised recommendations If the concern is an anergic reaction, the answer is "very quickly." At least 90% of the venom is released within 20 seconds of the sting, and 100% by one minute.