1. PRINCIPLE The presence of coliforms, faecal coliforms and aerogenic E. coli in water may be determined by means of the MPN procedure.

2. As indicated in section 1, the presence of coliforms, faecal coliforms and aerogenic E. coli in food and water may be determined by means of the MPN procedure.

3. No bite or scratch injury as site of entrance could be detected clinically or at autopsy, therefore aerogenic transmission in droplets from a household pet must be considered.

4. APPLICATION The Most Probable Number (MPN) method is applicable to the enumeration of coliforms, faecal coliforms and aerogenic Escherichia coli in water in sealed containers (including mineral and spring water) and prepackaged ice in accordance with Sections B12.001, B.12.004 and B.12.005 of the Regulations of the Food and Drugs Act.